Infants and young children present pain responses during intravenous cannulation procedures. In our unit, infants often cry and become alarmed during intravenous cannulation procedures. Our survey indicated that the severe pain rate of infants was 87.5%. and the influential factors were related to the lack of, first, technical standards for pediatric intravenous injections, second, use of analgesic, and third, assistive devices for fixation and distraction. This purpose of this project was to develop strategies to reduce the severe pain rate of infants in the unit. The proposed strategies include the development and promotion of pediatric intravenous technical standards and the recording of educational CDs, use of topical anesthetic agents for the epidermis, fixed limb assistive devices, use of multimedia to play music or cartoons, a painting room environment, and an audit mechanism. After the implementation of the project, the severe pain rate during intravenous injection was reduced from 87.5% to 32.0%. The results revealed that improving the injection skills of nurses, a child friendly environment, the use of assistive devices, and analgesics can reduce infant pain response during intravenous injection.