The education of Traditional Chinese medicine(TCM) has been developed and incorporated into the Objective Structured Clinical Examination (OSCE) for clinical skill assessment and training. Through the simulation of clinical situations, the clinical skill of TCM students is more effectively evaluated. This study aimed to explore the application and benefits of OSCE in acupuncture clinical training. In this study, data collection and analysis were conducted in a retrospective manner. Relevant materials of the acupuncture examination station of OSCE held by the Chinese Medicine Department of Changhua Christian Hospital from June 2013 to May 2016 were selected, including: analysis of the teaching plan of the acupuncture examination and the anonymous feedback and questionnaire of the candidates. During the study period, a total of 9 acupuncture test questions were developed, and all of the participating candidates were 49 (including 37 students and 12 first-year residents). The results of the study showed that the trainees confirmed the practicality of TCM OSCE for acupuncture clinical work. Because acupuncture is an invasive treatment and cannot be applied to standardized patients, this study suggests that in the future, it is necessary to further developappropriate acupuncture simulations according to the clinical situation of acupuncture; and it is necessary to pass the repeated tests to make the teaching test questions perfect. It is hoped that this study can provide reference for the future development of TCM OSCE and promote the transformation and maturity of TCM clinical education.