The pharmaceutical industry in China had a significant development during Republican China, through effective promotional strategies such as newspaper or magazine advertisements. Medicine advertisements not only stimulated the desire of purchasing, but also served as a tool to transfer the medical knowledge between“professional” and “everyday life”. Due to the target readers were not in the medical profession, medicine advertisements demonstrated how they accept or understand the professional medical knowledges. The content of medicine advertisement, revealed the social background, the public knowledge of medical concepts and the development of pharmaceutical industry in China. This paper takes Young Companions as a case study, to examine the medical concept and knowledge of the public in Shanghai, how professional medical knowledge was transferred to the public, thus influencing the everyday life, as well as how the public defined their own “professional” medical knowledge.