Introduction: Taekwondo was an important sport event to get gold medal in our country, its motion properties pertaining to the martial art with short-term high impact, in addition to possess a skilled attack techniques, physical quality must also have a good structure. Respiratory muscle fatigue had been confirmed in Endurance Sports and affected the sports performance, but it was still uncertain on the impact of Power Sports. Therefore, this study was aimed to explore the following issues: 1) the effects of respiratory muscle fatigue on the kicking performance of university Taekwondo athletes. 2) The effects of Taekwondo simulation match on respiratory fatigue muscles. Methods: This study was an exploratory and prospective designed, a total of 21 college Taekwondo athletes participated in this study as subjects. The first part of the experiment was carried out in December 2016 on the measurements for effects of respiratory muscle fatigue and kicking performance. The second part of the experiment was performed in March 2017 on the measurements before and after a simulation match. The measurement data included the maximal inspiratory mouth pressure and the degree of conscious breathing difficulties. Mann-Whitney U test with independent sample and Wilcoxon signed-rank test were used as statistical verification in order to compare the effects of respiratory muscle fatigue on the kicking performance and the effects on respiratory muscle before and after a simulation match of the subjects, with a standard of significance at p<.05. Results: In the first part of the experiment, after the kicking test, the variable of gender, Taekwondo age group and height group showed significant differences in the normal kicking state, yet the remaining variables were not significantly different. In the second part of experiment, the maximal inspiratory mouth pressure average decreased by 9.48% (before: 93.73±26.28cmH2O; after: 85.81±31.31 cmH2O, p=.001) after the simulation competition, while the degree of conscious breathing difficulties had significant increases (before: 0.67±0.80; after: 4.24±1.55, p=.03). Conclusion: The study shows that the variable of gender, Taekwondo age group and height affect the kicking techniques performance of university Taekwondo athletes, yet the single respiratory muscle fatigue has no significant effect on kick techniques performance. However, through the measurements before and after a simulation match, it was found that respiratory muscle fatigue will be produced and self-perceived breathing difficulty will increase.