This study explores that objective working time and subjective work stress for faculties in the universities of Taiwan. Teachers in the university are required to burden teaching, research and administration duties. Under different work environments and work conditions, what do they react about working time and work stress? What are obvious disparities between general university and university of technology? This article will intend to explore this topic. Based on the analyses of Taiwan Integrated Postsecondary Education Database (TIPED) for a representative sample from universities’ teachers, this study investigates the working time and work stress. The result will discover some, as follow: (1) There is no absolute relationship between work situation and working time, but the former has a significant impact on work stress. (2) Heavy working time of the teaching and over works of administrations will affect their intrinsic pressure but research work won’t cause too much pressure. (3) The work environment will be influential to teachers’ working time and work stress. This study result will increase a lot of knowledge and to realize teachers’ work condition, but also help to accumulate the literature of educational tracking in Taiwan.