In a randomized controlled, assessor-blinded clinical trial, 14 patients were treated with traditional acupuncture, 13 patients received laser acupuncture and 13 patients received sham laser acupuncture. Each patient received three sessions per week for four weeks on bilateral HT7 and LR3. The laser acupuncture therapy was performed with the Handylaser Trion (GaAlAs laser diode, 810 nm, 150 mW, pulsed waves), which delivered 0.375 J of energy (5 s), 1.5 J/cm2 in total. Heart rate variability (HRV), Center Epidemiologic Studies Depression Scale (CESD), Epworth sleepiness scale (ESS) and Pittsburgh Sleep Quality Index (PSQI) were evaluated before and after treatments. The reduction of PSQI score was significant both in traditional acupuncture group (p=0.021) and laser acupuncture group (p < 0.001) after treatment, but not in sham group (p=0.127). HRV, CESD and ESS scores were not improved significantly after treatment in all groups. The post-Hoc analysis showed the significant difference in PSQI score reduction between laser acupuncture group and sham group (p=0.015). Laser acupuncture and traditional acupuncture both seem to improve sleep quality in patients with insomnia, but there was no exceeding power of each group. Further study involving more subjects is needed to evaluate the therapeutic effect of acupuncture on insomnia.