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篇名
泌尿上皮失調與慢性發炎在反覆細菌性膀胱炎之角色探討
並列篇名
Explore the Roles of Urothelial Dysfunction and Chronic Inflammation in the Pathophysiology of Recurrent Bacterial Cystitis
作者 陳毓騏 (Yu-Chi Chen)陳忠賢 (Chung-Hsien Chen)吳振宇 (Chen-Yu Wu)郭漢崇 (Hann-Chorng Kuo)林嘉祥 (Victor C. Lin)
中文摘要

背景與目的:此文章之目標是探討泌尿道上皮失調及慢性發炎在反覆細菌性膀胱炎中的角色。方法:我們蒐集30 位在一年內發生至少兩次細菌性膀胱炎的女性病患。其中10為女病患有應力性尿失禁的問題但沒有任何膀胱刺激的症狀或泌尿道感染之情型。泌尿道上皮失能的指標如下:TUNEL- 細胞凋亡,Ki-67- 增值,tryptase staining - 肥大細胞活性,E-cadherin 以及zonula occludens-1- 間隙接頭蛋白表現。這些指標被用來評估泌尿道上皮失能的情形。結果:相較於對照組,實驗組的膀胱黏膜在免疫螢光染色下的E-cadherin 表現降低,但是在肥大細胞以及TUNEL 的表現增加。在IHC 染色下,E-cadherin, mast cell以及TUNEL 在反覆感染的族群與對照組有顯著的統計學上的意義。(p < 0.05) 用西方墨點法來分析phospho-p38 以及trypatse 的表現來證實發炎反應的發生以及Bax 蛋白質的表現來證實細胞凋亡的進程。相較於對照組,反覆泌尿道感染的族群的指標表現如下:phospo-p38(約3.6 倍),tryptase(約2.0 倍)及Bax(約2 倍)。結論:相較於對照組,在反覆性膀胱炎的患者,其慢性發炎以及細胞凋亡相關的表現在膀胱黏膜會增加。這些證據顯示在反覆性泌尿道感染的女性患者往往會表現出泌尿道上皮的失能以及慢性發炎。

英文摘要

Background and purpose: The aim of the study is to explore the roles of urothelial dysfunction and chronic inflammation in the pathophysiology of recurrent bacterial cystitis. Methods: A total of 30 women with recurrent bacteria cystitis at least twice in one year consecutivelywere enrolled in this study. 10 women with stress urinary incontinence without irritative bladder symptoms or any episode of UTI served as the control group. Urothelial dysfunction markers (TUNEL for apoptosis, Ki-67 for proliferation, tryptase staining for mast cell activity, E-cadherin and ZonulaOccludens-1 for junction protein expression) were assessed to explore the possibility of urothelial dysfunction in the bladder mucosa. Results: Compared to those in the controlled group, the bladder mucosa in the experimental group had reduced expression of immuno-fluorescent markers in detecting E-cadherin, but increased expression of mast cell and urothelial dysfunction (TUNEL). The expression of immune-histochemical (IHC) stain with E-cadherin, mast cell and TUNEL of recurrent group had significantdifference when compared to the controlled group, respectively. (p< 0.05) Western blot analysis for phospho-p38 and trypatse to confirm the inflammatory events and Bax protein expression to confirm the apoptotic process, which showed that the expressions of phospo-p38 (about 3.6 folds), tryptase (about 2.0 folds) and Bax (about 2 folds) increased in the recurrent UTI specimens compared with the normal control specimens. Conclusion: Increased expression related to chronic inflammation and apoptosis in bladder mucosa of the patients with recurrent urinary tract infection was observed when compared to those in normal control group. This evidence demonstrates that chronic inflammation and urothelial dysfunction are present in most women with recurrent UTI.

起訖頁 073-082
關鍵詞 細菌性膀胱炎泌尿道上皮失能免疫螢光染色Bacteria cystitisE-cadherinMast cellTUNELPhospo-p38Tryptase
刊名 輔仁醫學期刊  
期數 201506 (13:2期)
出版單位 輔仁大學醫學院
DOI 10.3966/181020932015061302002   複製DOI
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該期刊-下一篇 泌尿道結石在數位放射線影像中之可見度研究—假體實驗
 

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