Introduction: Muscle functional and cardiopulmonary fitness decrease with aging, the ability of the daily activities would reduce with lower limb strength decrease, and increased the risk of falling. Previous studies have reported that resistance training had efficiently prevented muscle strength lost of the lower extremities, however, it could induced muscle soreness after high-intensity resistance training. Recent studies have found long-term of progressive resistance training could reduce the magnitude of muscle damage and soreness; however, it is still unknown the effects of progressive eccentric-only training in the elderly on muscle function and functional fitness of the lower extremities. Purpose: The purpose of this study was to compare the changes in lower limb muscle function and functional fitness in response to progressive eccentric-only training and control group in old men. Methods: Sixteen old men were recruited in the study, and then were randomly assigned into progressive eccentric (ET, n=8) or control group (CON, n=8). The subjects in the ET groups were completed a 12-week training program using a leg extension machine. Maximal voluntary isokinetic contraction at 210°/s (MVC), rate of velocity development at 210°/s (RVD), 8 feet up and go, 30s chair stand up, and 6-min go were measured before, and after 12-week training. Data were analyzed by a two-way mixed design of analysis of variance. Results: After 12 weeks progressive strength training, there were significantly greater improvement in the performance of MVC, RVD, 8 feet up and go, and 30s chair stand up in the ET groups (p < .05). Compared to baseline, there was not any significant change in all measures after 12-week of observation for the CON group (p > .05). Conclusion: The results of this study showed that progressive eccentric training in old men could be effectively produced the same extent of improvements of MVC, RVD, and functional physical fitness. Furthermore, muscle soreness was not induced during the 12-week of ET regardless of progressive increased in training intensity. Therefore, progressive ET can be used one of effective and safe training programmes for old individuals or the results of this study can be provided as the suggestion for old individuals when they pursuit to promote their healthy or anti-aging.