Nurses played an important role in the palliative care team. Palliative care is provided by more and more departments in the hospitals. However, medical facilities in Taiwan have not provided systematic in-service educational courses in palliative care. This study aimed at exploring the in-service educational course needs in palliative care and analyzing the predictors of such needs among nurses. This was a correlational study using cross-sectional survey. The cluster random sampling based on the nurses’ proportion of units was used to select nurses from a northern medical center. The In-Service Education Scale in Palliative Care was distributed to the participants. Valid questionnaires were returned by 600 nurses, a response rate of 97.72%. The data were analyzed using descriptive statistics and inferential statistics with linear regression. The mean score of the In-Service Education Scale in Palliative Care among nurses was 3.95 (SD = 0.81). The level of needs ranked from high to low included “the preparation and care for dying process,” “symptom management,” “loss/grief/bereavement management,” “moral, legal, and ethical issues,” “pain assessment and intervention” and “communication,” “the professional stress, role, and function of nurses in palliative care,” “end-of-life care,” “cultural and spiritual considerations in palliative care,” and “introduction to palliative care.” In addition, multiple linear regression showed that the significant predictors for educational needs were religion, working experience, working unit, and willingness of participating in the in-service educational course in palliative care. This model accounted for 10.3% of the variances in nurses’ needs for the in-service educational course in palliative care. This study identified a high needs in the in-service educational course in palliative care among nurses. Hence, hospitals can design and plan the in-service educational course in palliative care based on actual demand as well as the nurses’ religion, working experiences, working units, and willingness.