Infection of double-lumen venous catheter not only affects quality of medical care but also threatens the patient’s life and safety. According to the analysis report from our infection control center, the infection rate of double-lumen venous catheter increased significantly from January to March in 2010, with one patient dying of sepsis. A task force was therefore set up for infection control. Our team checked catheter care conditions from nurses and primary caregivers. Using the “Technical Nursing Standards for Catheter Care” and “Catheter Care Checklist,” we found that the major causes of dialysis catheterinfection included: Insufficient knowledge on catheter care in nurses and primary caregivers, inadequate method of hand washing during catheter care, incorrect technique in catheter nursing care and inaccurate checking technique. After revising the “Technical Nursing Standards for Catheter Care”, define the “Evaluation of catheter wound”, we also promoted promote educational training of nurses and primary caregivers. Checklists were implemented and hand-washing equipment setup. After these measures were implemented, the infection rate of venous catheter was effectively reduced from 10.8‰ to 6.1‰, an improvement 43.5%. We concluded that our intervention was successful in improving the professional ability of nurses and effective in decreasing the infection rate of double-lumen venous catheter.