應用於食品安全監控感測器之電化學分析儀設計與實現

Design and Implementation of Electrochemical Potentiostat for Food Safety Monitoring Biosensors

本論文設計一個循環伏安式電化學分析儀作為電化學mRFP生物感測器之信號處理，此mRFP生物感測器是由台南大學生物科技系所開發，用以檢測食物溶液中是否具有毒性物質，所提出之電化學分析儀是利用嵌入式系統開發完成，以跨領域合作方式進行生醫感測器研究並利用嵌入式系統（EFM32微處理器）開發完成，並採用循環伏安法來分析溶液之檢體濃度變化。在實驗中透過微處理機EFM32輸出固定頻率的掃描訊號掃描待測物，觀測電流電壓圖並進行大範圍、高精度的分析。透過電流傳導的情況，可以利用Labview檢測待測物的各項參數並推測其性質。例如透過毒物檢測感測器來判斷檢測液中是否有具有毒性，並可進一步建立大數據資料庫。在系統驗證方面，我們的分析儀中的mRFP生物感測器針對不同濃度待測物的量測，系統對於感測器所量測到的電流變化進行分析，並與商用的分析儀所量測之結果進行比對，驗證本系統之特性。從實驗結果顯示，利用電化學方法來進行食物溶液中是否具有毒性物質之檢測，所需要之分析時間可以在幾秒中之內完成。

In this paper, we proposed a signal processing system, names as potentiostat, for electrochemical mRFP biosensor. The proposed system is used to test mRFP biosensor, which is designed with the laboratory of department of biological science and technology of NUTN, for food safety monitoring. The potentiostat is realized by using an embedded systems: microprocessor EFM32. The function of cyclic voltammetry is used to analyze the concentration of the samples. In the experiment, the microprocessor EFM32 outputs a fixed-frequency scanning signal to scan the biosensor, and measured the biosensor's current. This method can perform large-scale, and high-precision analysis. Through the conduction of current, Labview software is used to detect various parameters of the samples and to judge its results. For example, the poison detection sensor is used to judge whether the detection liquid is toxic or not, and establishing a big data database. In system verification, the proposed potentiostat are used to measure current changes of biosensor under different concentrations of the samples, and compares these results with the commercial potentiostat. From the experimental results, the testing procedure by means of electrochemical approach can be finished and obtained the results in a few seconds.