多環芳香烴化物對男性煉焦作業勞工生殖危害評估研究

Reproduction Hazards Evaluation for Male Coke Oven Workers Exposed to Polycyclic Aromatic Hydrocarbons

The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrityof coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to controlsubjects. The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as theexposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren.Analysis of sperm quality (concentration, motility, vitality, and morphology) was performedsimultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulkyDNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conductedto collect demographic and potential confounding data. The coke oven workers had lower percentagesof mobile sperm and sperm with normal morphology, and lower vitality than the control group, butthe difference was not significant. For DNA integrity, the coke oven workers had significantly higherconcentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048,respectively). However, DNA fragmentation percentages did not significantly increase as compared tothose in the subjects from the control group (p = 0.232). There was no correlation between sperm qualityparameters and DNA integrity indicators. Occupational exposure of the coke oven workers to PAHs wasassociated with decreased sperm DNA integrity.

本研究的目的為評估暴露於多環芳烴化物的煉焦作業勞工的精液品質與去氧核糖核酸（DNA） 之完整性，以某一鋼鐵工廠煉焦作業勞工為暴露組（N=35），及以行政人員為對照組（N=35）；以及以1- 羥基焦腦油作為多環芳烴化物的暴露指標。精子的品質分析包括濃度、活動性、活力及形態分析，並與精子DNA 完整性分析同時進行，包括DNA 片段化「變性」DNA 鍵結及8- 羥基-2- 去氧鳥嘌呤核甘分析。而以問卷調查收集個人基本資料與潛在干擾因子。煉焦作業勞工比對照組勞工有較低的精子活動性，活力與正常形態，但沒有達到統計上的顯著水準。而在DNA 的完整性方面，煉焦作業勞工比對照組勞工有顯著較高的DNA鍵結及8- 羥基-2- 去氧鳥嘌呤核甘濃度（p = 0.009 及 p = 0.048）。煉焦作業勞工的DNA 片段化沒有顯著高於對照組勞工（p = 0.232），精子品質與DNA 片段化沒有顯著關聯性。職業性暴露多環芳香烴化物會導致煉焦作業勞工之DNA 完整性顯著降低。