食品中番瀉苷（Sennosides）檢驗方法之精進   全文下載

Optimized Method of Sennosides in Foods

近年來，纖瘦體型蔚為風潮，致使不少宣稱可排便順暢、窈窕輕盈的產品陸續上市。觀察此類產品標示成分，其中不乏標示含番瀉苷成分者。長期食用含有番瀉苷成分的產品，輕者可能會產生依賴性，甚者還會危害身體健康。目前食品藥物管理署已針對膠囊、錠狀及茶包產品，公開「食品中番瀉苷之檢驗方法」（TFDAO0023.01），惟含番瀉苷之產品基質複雜，該建議方法無法全面適用，為縮短檢驗時間，精進檢驗方法，本研究嘗試使用超高效液相層析二維系統（UHPLC-2D）建立番瀉苷含量之檢驗方法，第一維液相層析使用C18管柱，以乙腈及0.1％甲酸溶液為移動相，於波長270nm進行檢視並選取目標吸收峰進入第二維分析，第二維液相層析使用Phenyl-Hexyl管柱，以甲醇及0.1％甲酸溶液為移動相，於全波長200－400 nm進行定性分析，並於波長270 nm進行定量分析。應用本檢驗方法進行標示或疑似含番瀉苷產品20件之檢測，結果所有檢體之二維層析圖譜均無干擾情形，顯示本研究建立之檢驗方法對於含番瀉苷產品之分析結果良好，可供各界進行食品中番瀉苷含量檢測之參考。

Nowadays, slim body becomes a trend. In consequence, various commercial products asserted for accelerated weight loss were hitting the market. Sennosides, a compound with functions of improving smooth defecation and weight loss may be found in claimed weight loss products. Due to sennosides may cause drug dependence and health risk for user, products contained sennosides should be avoided for long-term use. The analytical method for sennosides announced by Taiwan FDA, “Method of Test for Sennosides in Foods” (TFDAO0023.01), provided the quantitative analysis for sennoside A and sennoside B in capsules and related products. However, due to the complex matrixs of products, the method cannot be fully applied in all products. Thus, the aim of this study was to improve the previous method by the Two-Dimensional System of Ultra High Performance Liquid Chromatography (UHPLC-2D). A C18 column with acetonitrile and 0.1% formic acid as mobile phase was applied in the first-dimension of the system. Afterwards, selected target peak was analyzed in the second-dimension of the system. In the second-dimension of the system, Phenyl-Hexyl column with methanol and 0.1% formic acid was utilized as the mobile phase. The qualitative and quantitative analyses were detected at 200 - 400 nm and 270 nm, respectively. The surveillance results consisted of 20 commercial products which were labeled or suspected containing sennosides showed that chromatograms in all samples were not interfered. This method will be provided as reference to administrative authorities and public.