The Effects of Low Intensity Light Emitted Diode on Human Periodontal Ligament Cell and Mouse Cementoblast 68 Cell Line

The Effects of Low Intensity Light Emitted Diode on Human Periodontal Ligament Cell and Mouse Cementoblast 68 Cell Line

Objectives: The prevention of external root resorption caused by orthodontic tooth movement was endeavored by many researches. The photobiomodulation with low level dose light emitted diode (LED) is reported. The purpose of this study was to investigate the LED effects on the OCCM-30 and PDL cells proliferation. Materials and Methods: The cementoblast cell line (OCCM-30) and periodontal ligament (PDL) cells were cultured in the FX-5000 Compression System with the 0.255 kPA compression model for one hour; the experimental groups were treated with 860 nm low LED for 20 and 60 minutes. The control group did not receive the LED treatment. The proliferation of the OCCM-30 and PDL cells were evaluated by PrestoBlue assay. The cell number were counted and statistically calculated to compare the difference. Results: The results showed there were no morphologies changes between the control and experimental groups. The proliferation of the OCCM-30 and PDL cells showed no statistical difference between the control and experimental groups at different time interval of observation. Conclusion: It is concluded that the 860 nm LED did not induce the cell proliferation.