唐菖蒲組織培養之研究（一）花莖側芽之莖頂培養

Studies on the Tissue Culture in Gladiolus hybridus Hort. (Ⅰ) Shoot Tip Culture of Vegetative Lateral Buds on Flower Stems

本研究使用三個唐菖蒲品種：”Spic and Span”, ”white Friendship”和”Traveler”為臺灣經濟栽培品種。當花莖第1朵花後6遇，採取其上營養性側芽之莖頂，高300-500μ，在Knudson （1946）或稀釋之Murashige及Skoog（1962）培養基，莖6-8週後皆能形成小植株。若培養在添加BA0.4~0.8mg/e之培養基上，則在器內直接形成小球莖。小球莖再經多次分割培養，可達到快速增殖之目的。分割之小球莖組織塊，在添加BA0.8mg/e之Murashige及Skoog（1962）培養基上，每3~4週分割一次，連續分割培養，供為組織保存培養，如移植到無BA之培養基上，仍能分化葉，根及球莖。其在園藝上之應用會予與討論。

Three cultivars of Gladiolus, Spic and Span, White Friendship, and Traveler were used for this experiment. Shoot tips of the vegetative lateral buds were excised from flower stalks approximately six weeks after the first floret opened. The explants of 300 to 500μ in height were cultured on Knudson's (1946) or diluted Murashige and Skoog's (1962) medium in which the explants developed into plantlets within 6 to 8 weeks. Those cultured on the media supplemented with 0.4 to 0.8 mg/e of 6-benzyladenine (BA) neoformed cormel-like bodies. These bodies could be divided to accelerate multiplication. Stock tissue used for propagation was stored on the MS medium supplemented with 0.8 mg/e of BA and subdivision series proceeded in every 3 to 4 weeks. Leaves, root and corms differentiated gradually from stock tissue when transferred to the medium without BA. The possibility of horticultural applications through vegetative shoot tip culture of Gladiolus has been discussed.