芹菜體胚發生與植株再生

Somatic Embryogenesis and Plantlet Formation in Tissue Cultures of Chinese Celery

本試驗已建立本地種青梗芹菜（Apium graveolens L.）之細胞培養與植株再生體系。芹菜於無菌播種後，切取其子葉與下胚輸為培植體，培養於含kinetin及2,4-D各05mg/l的MS固體或液體培養基，可誘導胚性癒合組織之形成。固體或液體誘導之胚性癒合組織，繼續進行液體振盪培養，可建立芹菜懸浮細胞培養系統。源自胚性癒合組織或懸浮培養之體胚，培養於不合生長調節劑之MS培養基上，可發育成植株。

In this study, somatic embryogenesis and plant regenaration of Chenesis celery (Apium graveolens L.) in cell cultures was described. Callus cultures were initiated from hypocotyl or cotyledon explants of in vitro-grown Chinese celery and cultured with solid or liquid medium. The majority of induced calli was shown to be embryogenic when MS salts in combination with kinetin 05mg/l 2, 4-D 05mg/l, glutamine 100mg/l casein hydrolysate 500mg/l and sucrose 30g/l, were used in the solid or liquid callus induction medium. By using these embryogenic calli, a well grown embryogenic cell suspension culture was established and maintained in liquid medium of the same composition. The MS medium was the approprite medium for the initiation and development of somatic embryos from callus or suspension cultures. When transferred to a solid hormone-free medium the somatic embryos from either callus or suspension culture, developed into plantlets which could be transferred to soil and grown to maturity.