琴葉榕之組織培養繁殖

Tissue Culture Propagation of Ficus lyrata Warb

琴葉榕（Ficus lyrata Warb）之展開葉（25cm×18cm）的切塊（1cm×1cm），在MS之大量元素，配合Nitsch and Nitsch之微量元素之MSNN基本培養基，分別添加IBA 1或2.5 mg/e與BA 5mg/e；IBA 1mg/e，BA 2.5mg/e或含IAA 1mg/e, BA 5mg/e之B5培養基，都能有效誘發癒合組織並有不定芽分化。誘生的不定芽經由含BA 1mg/e與GA 0.5~2mg/e之WPM培養基培養，可促使枝條發生細密分校的現象，及在伸長的葉片上或葉柄上有大量不定芽發生。繁殖所得的芽軍，分切培養於含BA 1mg/e之WPM培養基，可萌發伸長成正常枝條。這些枝條可以用不含生長素的基本培養基培養而促使發根長成小植株。小植株移出試管種植，成活率高達98%以上。從原始培植體至長成小植株約需時3~4個月。

Leaf-squares (1cm) of Ficus lyrata were cultured on MSNN medium containing Murashige and Skoog (1962) macroelements and Nitsch and Nitsch (1969) microelements supplemented with 1 or 2.5 mg/e of IBA and 5mg/e BA; or with 1mg/S IBA and 2.5mg/e BA; and also on B5 (Gamborg et al., 1968) basal medium supplemented with I mg/e IAA and 5mg/e BA. All media effectively induced callus and regenerated adventitious buds. Subculture of regenerated buds on WPM (Lloyd and McCown, 1980) medium supplemented with 1mg/e BA and 0.5-2.0mg/e GA resulted in the production of multiple slender shoots and additional adventitious buds on the petiole or leaf-blade. Divided clusters of buds cultured on WPM medium containing 1mg/S BA gave normal shoots. These shoot cuttings could be easily rooted in auxin free WPM basal medium. Rooted plantlets were successfully transplanted to equal parts of vermiculite and peat moss mixture. It took 3-4 months from the starting of culture to regenerated plantlets ready for transplanting.