亞太地區香蕉種原複製保存中心的建立與運作

Establishment and Operation of the Asia and Pacific Banana Duplicate Collection at TBRI

國際香蕉改良協會（INIBAP）於1985年在比利時魯汶大學設有香蕉種原保存及轉輸中心，收集並以組織培養法保存全球香蕉種原1050個。為防意外確保種原之安全，乃於1993年與台灣香蕉研究所簽署合作備忘錄，在本所設立亞太地區香蕉種原複製保存中心。並於同年接欣首批種原153個；1994年繼續收到從新畿內亞收集之香蕉種原271個。合計保存424個亞太地區種原。這些種原以莖端組織培養法在最低生長條件（16°C溫度及每天12小時lOOOlux光照）下進行保存。每品系保存6至12支試管，每月進行檢查，每年更新改良型MS培養基一次。保存庫裝有溫度控制保安系統，預防因機器故障造成溫度過高或過低，以致傷害種原。本中心之建立，具有確保亞太地區香蕉種原之安全，及增進國際間合作，共同保護珍貴種原等目的。

The INIBAP Musa Germplasm Transit Centre (TIC) located at the Catholi'c University of Leuven, Belgium was established since 1985. Currently, it holds about 1050 accessions of Musa germplasm by in vitro method. For reason of safety, the Asia and Pacific Banana Duplicate Collection at the Taiwan Banana Research Institute (TBRI) was established after signing the MOU by the representatives of the two Institutes in 1993. The first batch of 153 accession from Asia-Pacific region was received by TBRI in the same year. In 1994, another 271 accession collected from Paupau New Guinea was duplicated and shipped to TBRI for storage. Shoot-tip tissue culture method is used to conserve the duplicate collection at TBRI. The culture is maintained at the minimal growth condition with 16°C and under the light intensity of 1000 lux for 12 hours each day. Each accession is kept in 6 to 12 test tubes. All accessions will be inspected for contamination regularly every month, and subcultured to fresh modified MS medium once a year. Safety device was set to prevent damage of germpalsm due to break down of the cooling system. The establishment of the Banana Duplicate Collection at TBRI provides an exemplary institutional committment to the intemational effort to conserve Musa germplasm.