蘋果汁中PATULIN分析方法之探討（一）－－薄層層析法與氣相層析法的比較

Studies on the Methodology of Patulin Analysis in Apple Juice -- Comparison of the Thin Layer Chromatography and Gas Chromatography

本實驗探討以薄層層析法（TLC）及氣相層析質譜法（GC/MS）分析蘋果汁中patulin含量之優劣。結果顯示薄層層析法之偵測極限（100 α g/1）高於patulin的安全限量（50 α g/1），不適用於定量patulin 將patulin衍生為patulin acetate再配合GC分析及比對檢量線，可準確定量，回收率90%。偵測極限10 α g/1。實驗亦發現若直接於樣本中進行醯化衍生反應（acylation derivation）可提高回收率。

Analytical methodology for patulin contents in apple juice was studied by the comparison of thin layer chromatography (TLC) and gas chromatography with mass spectrometry (GC/MS). Results indicated that the determination limit of TLC (100 μ g/l) was above the safe contamination level (50 μ g/l) of patulin. A method for the analysis 0f patulin in apple juice was developed using GC of patulin acetate. Patulin acetate can be measured down to a levl of 10 μ g/l with 90% recovery. Results also indicated that conversion of patulin to patulin cetate in aqueous samples before extraction can increase the extraction and recovery efficiency.