芋原生質體分離與融合之研究

Studies on ths Protoplast Isolation and Fusion of Taro (Colocasia esculenta Schott)

高雄1號芋塊莖質優豐產但採苗率低，紅梗芋塊莖品質差，但採苗率 高，因芋屬無性繁殖作物，無法行有性雜交，故本試驗嘗試分離芋原生質體，探討芋原生質體融合之可行性，以作為改良芋生產之依據。芋葉片或葉柄組織以10%mannitol，0.2% CaCl2‧2H₂O，1% cellulase，0.5% macerozyme之酵素溶液處理10小時可獲得最多量之原生質體。純化芋原生質體，以15～17.5%的蔗糖溶液效果最佳。芋原生質體經以50%PEG 6000加入7mM CaCl₂ 2H₂O的誘融劑處理後，約可使6.2%原生質體發生融含。

For improving the low seedling rates but high quality in Kaoshiung 1, and high seedling rate but low quality in red-petiole taro varieties, the technologies of taro protoplast isolation and fusion were developed to improve the production of taro. Maximum number of protoplasts was obtained when taro leaves or petioles were treated with enzyme solution containing 10% of mannitol, 0.2% of CaCl₂‧ 2H₂O, 1% cellulase and 0.5% of macerozyme for 10 hours. Further purification of protoplast could be achieved in 15~17% of sucrose solution. Best fusion inducer was found when 50% of PEG 6000 was added to 7mM of CaCl₂•2H₂O.