| 中文摘要 |
使用Red Sunblaze和Royal Sunblaze兩品種迷你玫瑰（Rosa chinensis Jac. 'minima'）的單節組織培養時，保持母體莖組織對新生枝條之生長有助益。將莖段平放、直插或斜插，所長出新枝條的鮮重和乾重，比將莖段倒插所長出的新枝條為重。利用母株開花枝條上端五節位莖段培養時，腋芽之發芽率及新生腋芽之鮮重與乾重比利用第六或七節的莖段培養者高且重。在5〜40μ mol m-2 s-1光度範圍內，較高強度環境下培養者，新梢生長、成熟及老化都較快。而最適於初代培養的光度為20μ mol m-2 s-1。再以25種不同濃度的Benzyladenine（BA）和Naphthalene acetic acid（NAA）組合進行8個品種之單節培養，顯示單節培養對培養基的選擇性與品種有關；如Red Sunblaze, Baby Katie, Central Gold, Green Ice, Orange Sunblaze和Sequoia Gold等品種適於生長在含NAA 0-0.lmg l-1和BA O.lmg 1-1的培養基。而Lavender Jewel和Royal Sunblaze品種，則在含NAA O-O.lmg 1-1和BA l.Omg 1-1的培養基中生長最好。每一個長、寬及高度分別為7.5、7.5及10cm，之容器（GA-7型容器）以培養9～16莖段時，枝條增生的效率最高。 |
| 英文摘要 |
The growth and establishment of new lateral shoots of miniature roses (Rosa chinensis Jacq. minima, cvs., Red Sunblaze and Royal Sunblaze), from nodal explants was enhanced by the presence of parental stem tissue. Fresh and dry weight of shoots from a vertical, inclined or horizontal explant were greater than that those from an inverted explant. Fresh and dry weight of shoots and rate of lateral bud sprouting from explants from the upper five nodal segments were greater the lower two nodal segments. Under 5 to 40 μmol m-2s-1 lighting condition, the higher the photosynthetic photon flux (PPF) was the more quickly explants grew, matured and senesced. Initial growth was favored at a PPF of 20 μmol m-2 S-1. Twenty five combinations of 6-benzyladanine (BA) and 1-naphthale- neacetic acid (NAA) concentrations were tested for eight cultivars using selected explant and PPF conditions. Choice of medium for explant growth was dependent on cultivar: optimal growth of 'Red Sunblaze', 'Baby Katie', 'Central Gold', 'Green Ice', 'Orange Sunblaze', and 'Sequoia Gold' were achieved on a medium contain ing NAA at 0-0.1 mg 1-1 plus BA at 0.1 mg 1-1. However, the best growth response of 'Lavender Jewel' and 1 Royal Sunblaze' wasa achieved with NAA at 0-0.1 mg l-1 plus BA at 1.0 mg 1-1. Shoot proliferatoin was maximied when 9-16 explants were placed in each vessel. |
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