光和蔗糖對玫塊花組織培養培殖體光自營生長的影響

Effect of Photosynthetic Photon Flux Density and Sucrose on the Photoautotrophy of Rose Explants in Vitro

Samantha'和'Landora'的枝梢培殖體，在高光度（70μmol m^(-2)sec^(-1)）下培養，其生長量皆大於低光度（35μmol m^(-2)Sec^(-1)）下的生長量。而在高光度下，培殖體以培養於含蔗糖濃度為15g/l生長量最高。約40% 'Samantha'培殖體可在高光下之無糖培養基中自營生長，但在相同的條件下，'Landora'培殖體僅50%可活到第三週。）培養基的蔗糖濃度為15g/l時，'Samantha'和'Landora'的培殖體在高光度的環境下培養4週後，容器內CO2濃度的平衡點在明期最低可達0 ppm；而在低光度環境下，培養'Samantha'或'Landora'容器內CO2濃度的平衡點在明期最低分別為130 ppm或420 ppm。培養基的蔗糖濃度為30g/l時，'Samantha'或Landora'的培殖體在高光度的環境下培養4週後，容器內CO2濃度的平衡點在明期仍高達620 ppm或830 ppm以上。以不含蔗糖培養基培養'Samantha'培殖體的容器內，在高光度下明期與暗期CO2濃度平衡點之差值，隨時間的增加而增加，培養4週後容器內CO2濃度的平衡點在明期最低可達100 ppm；但以不含蔗糖培養基培養'Landora'培殖體，在高光度或低光度下，培養容器內明期和暗期的CO2濃度變化小。

The growth of the explants of Rosa hybrida cvs. 'Samantha' and 'Landora' shoots cultured under higher (700 μmol m^(-2) sec^(-1)) photosynthetic photon flux density (PPPD) was better than those cultured under lower (35 μmol m^(-2) sec^(-1)) PPFD. Under high PPPD, explants collared on medium, containing 15g/l sucrose, got the highest fresh weight. About 40% of 'Samantha' explants cultured under high and medium, without sucrose, survived at the end of the 4(superscript th) week. However, under the same condition only 50% of 'Landora' explants survived at the end of the 3(superscript th) week. After the shoot explants of 'Samantha' and 'Landora' were cultured under high PPFD and medium, containing 15g/l sucrose for 4wks, the stable concentration of CO2 in culture vessel reached 0 ppm during light period. However, under tow PPFD, the stable concentration of CO2 in vessel, which 'Samantha' or 'Landora' explants were cultured, was 130 or 420 ppm, respectively. When the sucrose in the medium was increased to 30g/t, 'Samantha' or 'Landora' explants were cultured under high PPFD the CO2 concentration in vessel was high to 620 or 830 ppm. When the explants of 'Samantha' cultured on medium without sucrose nod under high PPFD the CO2 concentration difference between light and dark periods increased as well as the days of culture extended. Furthermore, after a four-week culture, concentration during light time was as be as l00 ppm only. However, 'Landora' explants cultured on medium without sucrose, no differences were observed in the CO2 concentration between light and dark periods, whitchever explants were under high or low PPFD.