凍結切片、冷凍脫水、乾安置及放射能顯像技術聯合應用於數種園藝植物材料之研究

The Combined Usage of Freezing Sectioning, Freeze-dried Frozen Sections, Dry Mounting and Autoradiography in the Studies of Some Horticultural Materials

應用放射能顯像術，聯合可擴散性物質之組織切片處理，對放射性核種標識之養份，於植物某部份器官或果實內分佈詳情，以及其代謝途徑，或用於植物保護之藥物殘留狀況之追索，某種有害金屬之染污及其積集情形等，均可藉此求得一深入了解。本研究以草莓及番茄果實，葡萄主莖，及鮑魚菰子實體等為試材，分別使其吸收³⁵S，⁹⁰Sr，¹⁴C及²⁰³Hg等核種，事後循凍結切片，冷凍昇華脫水，採併置法或包捲法乾安置於放射能顯像乳膠，由所生成像斑，以研究各放射性核種存在於不同組織內之詳細實況。所得結果顯示，不論為細胞之組織構造，及其精微明晰之放射性核種於細胞間之分佈圖像，均證實所採用方法，對前述問題能供給頗為詳盡之解惑。

The combined usage of autoradiography and the histological processing of diffusible substances was adopted in studying the distribution in detail of some labelled nutrients within a particular plant organ or in a portion of a plant or a fruit and the metabolic pathways in the whole plant. The techniques can also be used for studying the residual chemicals for plant protection as well as the accumulative status of some toxic metals in plant body due to contamination. Strawberry and tomato fruits, stem of Vitis venifera and cap of Pleurotus Florida were used as the materials and ³⁵S, ⁹⁰Sr, ¹⁴C and ²⁰³Hg were used as the tracing radionuclides in this research. After absorption, the histological processing of the samples was carried out by frozen sectioning and sublimate-dried. The autoradiography was achieved by dry mounted onto the Fuji ET-2F nuclear emulsions through apposition or wrapping method. The autoradiograms induced by the radionuclides displaying in various tissues in cellular level indicated the detail true. Results obtained in this study also revealed the acceptable histological quality and resolute autoradiograms of some radionuclides which may confirm the feasibility of the techniques in solving the problems mentioned above.