大蒜莖頂生長點培養

Studies on the shoot Meristem Culture of Allium satium L.

培養大蒜生長點圓錐體之適宜培養基為最初一個月在（1公升中含有下列各成份三分之一含量）Ca(NO₃).₂4H₂O 5l0mg; KC1 240mg; KH₂PO₄ 120mg; MgSO₄7H₂O 720mg; NH₄NO₃ 180mg; Na₂-EDTA 37.3mg; FeSO₄7H₂O 27.8mg; micro elements of Murashige & Skoog's medium; adenine 5mg; biotin 0.01mg; Ca-pantothenate lOmg; cystin lOmg; casein hydrolysate lmg; myo-inositol O.lmg; nicotinic acid lmg; pyridoxin lmg; thiamin lmg; glucose 30g.） 溫度為25°C，0-150 lux光強下培養，而後移至以上之全含量培養基加O.l-1ppm之NAA，在25°C，500-2200lux光照下培養，可得較好之苗體。從100-200μ高之生長點圓錐體培養所得之苗體均無毒素病徵。

The study on shoot meristem culture of Allium sativum L. was conducted. The preparation of the fall strength culturing medium was as the following: Ca (NO₃)₂ 510mg; KC1 240mg; KH₂PO₄ 120mg; MgSO₄ 7H₂O 720mg; NH₄NO₃ 180mg; Na₂-EDTA 37.3mg; FeSO₄ 7H₂O 27.8mg; micro elements of Murashige & Skoog's medium; adenine 5mg; biotin O.Olmg; Ca-pantothenate 10mg; cystin 10mg; casein hydrolysate lmg; myoinositol O.lmg; nicotinic acid lmg; pyridoxin lmg; thiamin lmg; and glucose 30gm in one liter. The 100 to 200μ height of garlic shoot apical meristems were initially cultured in 1/3 strength of the culturing medium, under 25°C, and 0 to 150 lux light intensities for one month, and then were transferred to the full strength of the medium containing 0.1 to 1 ppm of NAA; under 25°C and 500 to 2200 lux light intensities. The plantlets obtained were strong and no virus symptomes.