黃紋萬年麻之組織培養

In vitro propagation of Furcraea foetida

本試驗以黃紋萬年麻花莖上之小鱗莖頂芽及鱗片為培植體，置於MS基本培養基進行初代培養。經12週初代培養，頂芽培植體形成芽體百分率達100%，顯著高於鱗片培植體；而平均每個培植體產生之芽體數，則以培養基含1m／l NAA及4mg／l BA的3.8個最高，其次為2mg／l BA之3.0個。由鱗片培植體誘致之芽體，則易產生不具斑紋之綠葉株。繼代培養以MS培養基含1mg／l NAA及2mg／l TDZ處理，對芽體增殖效果最佳。瓶苗移植出瓶，以株高4-8cm植株出瓶成活率高且相對生長速率最高。而瓶外生根則以1000mg／l NAA粉衣處理對發根效果最佳。

The apices or scales from the bulbils of Furcraea foetida cv. Striata were used as the explants for in vitro culture. Results indicated that the efficiency of shoot formation from bulbil-apex explants was higher than that from scale explants. An average of 3.8 shoots was formed from each bulbil-apex explant cultured in the MS medium supplemented with NAA 1 mg/l and BA 4 mg/l. In comparision, an average of 3.0 shoots was formed in the MS medium supplemented with BA 2 mg/l, after twelve weeks inoculation. The homogeneous green plantlets frequently regenerated from striated-scale explants. The optimum timing for subculture was 5-6 weeks after inoculation and shoots proliferation was significantly enhanced in the MS medium supplemented with NAA 1 mg/l and thidiazuron (TDZ) 2 mg/l. The optimum height for plantlets transferred from the test tube to soilless mixes was 4-8 em, which resulted in high survival rate and high relative growth rate. For root formation in vivo, plantlets treated with NAA 1000 mg/l was found to have the best result.