臺灣產保育類無霸勾蜓之遺傳多樣性

Genetic Diversity of the Protected Jumbo Dragonfly Anotogaster klossi Fraser (Odonata: Cordulegastridae) in Taiwan

無霸勾蜓為臺灣體型最大，也是唯一的保育類蜻蛉目昆蟲。雖然名列保育物種受到較多關注，然而相關基礎生物學研究卻不多。本研究為瞭解臺灣產無霸勾蜓的遺傳多樣性與族群分化，於全臺8樣點採計15樣本。細胞核（ITS1）與粒線體（COI）基因序列分析結果，區分出2個ITS1單倍型與8個COI單倍型，地理分布上無明顯遺傳分化現象。粒線體COI序列之單倍型多樣性（h）與核苷酸多樣性（π）各為0.895與0.0052，且其變異形式無明顯偏離中性假說。以上結果初步呈現臺灣產無霸勾蜓遺傳性質，可作為後續保育研究之參考。

The jumbo dragonfly Anotogaster klossi Fraser is the largest and the only protected Odonata species in Taiwan, but there is little known biological information for conservation efforts. In order to understand the genetic differentiation and diversity of this species in Taiwan, we sequenced both the nuclear (ITS1) and the partial mitochondrial (COI) segments from 15 samples collected in eight localities across Taiwan. A total of two ITS1 haplotypes and eight COI haplotypes were identified, and no obvious genetic differentiation was found across the examined geographic range. Haplotype diversity (h) and nucleotide diversity (π) of the mitochondrial COI segment were 0.895 and 0.0052, respectively. The sequence variation patterns of the population showed no significant difference from neutrality. These results reveal the genetic backgrounds of A. klossi in Taiwan and provide useful information for its conservation.