辣椒素酯類物質補充對運動期間能量代謝與發炎的影響

Effects of oral capsinoids supplementation on energy metabolism and inflammation during exercise period

緒論：研究指出CH－19甜椒萃取物辣椒素酯類物質（capsinoids, CSN）在安靜狀態期間具有增加能量消耗與脂肪氧化作用。然而，尚無人體研究證實運動前CSN補充是否有助於改變後續高強度運動期間能量使用偏向脂肪氧化，同時減緩運動發炎指標產生。因此，本研究目的主要探討CSN補充對運動期間能量代謝與發炎指標的影響。方法：以9名男性規律運動者為本實驗之受試對象（年齡：20.4±0.2歲；身高：170.6±3.1公分；體重：63.8±3.7公斤；身體質量指數：21.7±0.7kg／m2；最大攝氧量：48.2±2.9ml／kg／min），採用隨機單盲交叉設計，兩次試驗至少間隔七天，運動前60分鐘進行輕熱量早餐及30mg CSN或安慰劑膠囊補充，隨後進行肝醣耗竭運動挑戰，在補充前、運動期間及運動後分別收集氣體樣本分析呼吸交換率，採集的血液樣本分析葡萄糖、非酯化脂肪酸、甘油、細胞激素介白素－6（interleukin-6, IL-6）、介白素－1β（interleukin-1 beta, IL-1β）及腫瘤壞死因子－α（tumor necrosis factor-alpha, TNF-α）等指標的濃度。結果：CSN補充後運動期間第30分鐘及運動後之非酯化脂肪酸與甘油濃度明顯低於安慰劑試驗（p＜.05），然而，兩種試驗的呼吸交換率無顯著差異；同時，兩種試驗的IL－1β、IL－6和TNF－α等發炎指標濃度皆無顯著差異。結論：由非酯化脂肪酸與甘油資料顯示CSN補充增加後續運動期間脂質利用，但是無法改變運動期間全身脂肪氧化程度。因此，推論CSN補充無助於後續運動期間全身脂肪氧化及IL－1β、IL－6和TNF－α等發炎指標的減緩。

Introduction: The literatures review on capsinoids (CSN) has been shown that CSN extracted from CH-19 sweet pepper have increased resting energy expenditure and fat oxidation in animal and human studies. However, little human study was found regarding to oral CSN supplementation can alter energy source reliance on fat oxidation and attenuate inflammatory markers during high-intensity exercise with glycogen depletion protocol. Therefore, the purpose of this study was to determine effects of oral CSN supplementation on markers of energy metabolism and inflammation during exercise period. Methods: Nine subjects (age: 20.4 ± 0.2 years; height: 170.6 ± 3.1 cm; weight: 63.8 ± 3.7 kg; BMI: 21.7 ± 0.7 kg/m2 ; VO2 max 48.2 ± 2.9 ml/kg/min) with regular activity habit were recruited to perform this cross-over study design, all subjects were randomly assigned into CSN or placebo treatment, separated by a 7 days washout period. Every subject performed a single bout of cycling exercise with glycogen depletion protocol after 30 mg CSN or placebo supplementation accompany by 300 kcal normal diet. Gaseous exchange sample were taken and blood samples were obtained for measures on respiratory exchange rate, glucose, non-esterified fatty acid, glycerol, interlukine-1β, interlukine-6 and tumor necrosis factor α prior to supplementation, before and immediately after exercise. Results: There were no differences in respiratory exchange ratio between CSN and placebo trials. Both glucose responses of CSN or placebo trials were decreased during exercise periods. However, no significant differences were found between CSN and placebo at time points. Lower responses of plasma non-esterified fatty acid and glycerol responses were found in CSN comparing to placebo after 30-min point during exercise period. Additionally, there were no differences in cytokines with interlukine-1β, interlukine-6 and tumor necrosis factor α between CSN and placebo trials. Conclusion: Oral CSN supplementation could stimulate lipid utilization, indicated lower response on plasma non-esterified fatty acids and glycerol during exercise, but did not result to significant change in fat oxidation. Therefore, there is as yet no evidence in the present study to demonstrate the CSN benefit on enhancing whole body fat oxidation and attenuating inflammation during high intensity exercise challenge in young athletes.