| 中文摘要 |
人體隨著年齡增長，老化現象也隨之產生，尤其皮膚的老化更為人們最重視的顏面，因此抗老化的產品及方法開發從古至今持續著備受關注。引起皮膚老化成因有許多，其中紫外線所導致之皮膚光老化，為日常中最普遍的原因。過去文獻指出葛根素屬於天然的異黃酮類化合物，在不同疾病上分別具有對抗發炎、抗氧化及抑制DNA損傷的功能，因此本篇將利用體外試驗探討葛根素是否可以抑制皮膚因紫外光導致光老化的情形，然而葛根素雖具有良好的藥理作用，單純的葛根素卻於人體中的生物利用率低，因此，在本文中我們將比較純葛根素與脂質體葛根素的抗炎或抗凋亡作用，希望可以藉由將葛根素包覆於藥物載體提升其生物利用率。由初步結果顯示，經由ANOVA統計分析找到製備微脂體最佳比例為E6C2T2P2，載藥量：16.23%，粒徑約在140 nm。在細胞實驗，葛根素本身具有改善HaCaT細胞因UVB導致的細胞凋亡及發炎反應的趨勢，然而將葛根素包覆於微脂體後，卻不具有相同的改善效果，因此未來可再針對包載葛根素的藥物載體進行研究及改善。
With the increase of age, the aging phenomenon also occurs, especially the aging of the skin is the most important face of people. Therefore, the development of anti-aging products and methods has been receiving much attention since ancient times. There are many causes of skin aging, and the photoaging of the skin caused by ultraviolet rays is the most common cause in daily life. In the past, it was pointed out that puerarin belongs to natural isoflavones and has anti-inflammatory, anti-oxidative and DNA-inhibiting functions in different diseases. Therefore, this article will investigate whether puerarin can inhibit skin light caused by ultraviolet light in cell model. However, puerarin has a good pharmacological effect, but it has low bioavailability in human body. Therefore, we will compare the anti-inflammatory or antiapoptosis effects of pure puerarin to that of liposomal puerarin. It is hoped that the bioavailability can be enhanced by coating puerarin in a drug carrier. Preliminary results showed that the optimal ratio of preparation of liposome was E6C2T2P2 by ANOVA statistical analysis, the drug loading was 16.23%, and the particle size was about 140 nm. In the UVB-stimulated keratinocyte experiment, puerarin tends to improve the apoptosis and inflammatory responses in HaCaT cells. However, liposomal puerarin does not have the same improvement effect, so it can be further research and improvement of drug carriers containing puerarin. |
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