蝴蝶蘭分生組織培養去病毒及以短暫浸漬系統進行快速繁殖

Virus Elimination through Meristem Culture and Rapid Clonal Propagation by Temporary Immersion System in Phalaenopsis

本研究旨在探討蝴蝶蘭分生組織培養去病毒技術及無病毒植株之快速繁殖。用不同大小之莖頂分生組織培養，結果較大培植體有較高的存活率。圓錐體帶2片葉原體之Phalaenopsis Sogo Yukidian 'V3'得50.0%及圓錐體帶4片葉原體之Doritaenopsis Queen Beer得43.3%之存活率。然而以圓錐體帶1片葉原體之分生組織培養有較高的去病毒效率；Phal. Sogo Yukidian 'V3'有71.1%及Dtps. Queen Beer有50.0%去病毒率。經由生物晶片檢測之無病毒植株短縮莖培植體進行營養系快速繁殖之結果，短暫浸漬系統（temporary immersion system；TIS）之效率優於半固體培養。Phal. Sogo Yukidian 'V3'於浸漬頻率為每4小時1次，每次10分鐘循環培養之相同培養容器中，由1個無病毒短縮莖為起始，經120天培養獲得154個再生芽體。Dtps. Queen Beer則可獲得42.8個再生芽體。短暫浸漬系統應可作為無病毒健康蝴蝶蘭種苗之快速生產之用。 This study aimed at virus elimination and rapid clonally propagation of virus-free Phalaenopsis plants via meristem culture. Using larger meristem-tips as explants, resulted in higher survival rates than using smaller ones. The survival rate of Phal. Sogo Yukidian 'V3' explants with a dome and 2 leaf primordia was 50.0% and that of Doritaeuopsis Queen Beer explants with a dome and 4 primordia was 43.3%. However, smaller explants were more effective for virus elimination. Using explants with a dome and 1 primordium each, we obtained 71.1% of Phal. Sogo Yukidian 'V3', and 50.0% of Dtps. Queen Beer plantlets virus-free. For rapid clonal propagation of the short-stem virus-free plantlets passed bio-chip screening, temporary immersion system was more efficient than semi-solid culture. For shoot proliferation, the initial virus-free short-stem explants were cultured in the temporary immersion system with a frequency cycle of 10 minutes of immersion time, 6 times per day. After 120 days of culture, each of Phal. Sogo Yukidian 'V3' explants multiplied into 154 shoots, and each of Dtps. Queen Beer explants multiplied into 42.8 shoots on average. The temporary immersion system may be useful for rapid mass propagation of virus-free phalaenopsis.