菊花葉片培養與植株再生

In Vitro Leaf Culture and Plantlet Regeneration in Dendranthema Cultivars

以'Blush Hawaii'菊花及其他品種試管苗為材料，將葉片橫切培養，觀察培植體不定芽的形成，並將所產生不定芽經發根馴化後移至田間，觀察產生變異之現象。不定芽的再生以MS為基本培養基，添加TDZ及Kinetin，TDZ及2iP，TDZ和BA。大部份處理均可誘導產生不定芽，其再生比率為026.3%；而平均每個培植體產生的不定芽數為019.5個。以MS+0.5mg／lTDZ+1mg／l2iP培養基比較17個品種的再生，其中‘橙精興之華’、‘黑咖啡’、‘阿萊粉’、‘荷蘭白’、‘寒紅梅’、'Regal Westland'、'Cocarde'、'Tiger'、'Paso Doble'、'Improved Mundial'、'Salmon Impala'、'Fiji'等12個品種均可產生不定芽。以‘寒紅梅’所產生的不定芽數最多，每個培植體平均產生8.5個不定芽，再生比率為20%；'Fiji'品種次之，每個培植體平均產生7.3個不定芽，再生比率為18.8%。 The purpose of this study was to establish a regeneration protocol from in vitro lamina culture of chrysanthemum shoots. In vitro shoots of Blush Hawaii and other cultivars were used as starting materials for leaf explant source for adventitious shoot formation. The established plantlets were also grown in the field to evaluate somaclonal variation. Murashige and Skoog medium was used as basal medium supplemented with various concentrations of thidiazuron (TDZ), kinetin, 2isopentenyl adenine (2iP) and benzyladenine (BA). Adventitious shoots were regenerated from most treatments ranging at 026.3% and average of 019.5 shoots per explant. One medium, MS+0.5 mg/l TDZ+1 mg/l 2iP, was chosen for comparison of 17 varieties for regeneration response. Among tested cultivars, a total of 12 cultivars regenerated shoots, including Cheng-Jin-Hsin-Tze-Hua, Hei-Ka-Fei, Ah-Lai Pink, Holland White, Cold Homae, Regal Westland, Cocarde, Tiger, Paso Doble, Improved Mundial, Salmon Impala and Fiji. The cultivar Cold Homae was most responsive, with regeneration rate of 20% and average 8.5 shoots per explant. Fiji responded next, with regeneration rate of 18.8% and average 7.3 shoots per explant.