多層全像非軸向掃描狹縫共軛焦螢光顯微鏡

Development of Functionalized Electrospun Nanofiber Mat for Sweetener Production from Mogrosides

本研究提出了一種非軸向掃描多焦平面共軛焦顯微鏡系統，結合了多層體積全像光柵和狹縫陣列檢測，可同時擷取不同深度的光學切片影像。顯微鏡系統中配置多層體積全像光柵，於樣本內的兩個或多個焦平面中選擇性地產生軸向焦點，然後使用共焦狹縫孔徑同時將多個平面成像到CCD的感測區域。本文說明了系統設計、實施方法和實驗數據，證明了這種顯微鏡系統不需利用任何軸向掃描裝置即能夠獲得螢光標記的標準微球和組織樣本的光學切片多焦平面影像。 A non-axial-scanning multi-plane microscopic system incorporating multiplexed volume holographic gratings and slit array detection to simultaneously acquire optically sectioned images from different depths is presented. The proposed microscopic system is configured such that multiplexed volume holographic gratings are utilized to selectively produce axial focal points in two or more planes inside the sample, and then to use confocal slit apertures to simultaneously image these multiple planes onto corresponding detection areas of a CCD. We describe the design, implementation, and experimental data demonstrating this microscopic system's ability to obtain optically sectioned multi-plane images of fl uorescently labeled standard micro-spheres and tissue samples without scanning in axial directions.