快速大量研究蛋白質的利器：高通量西方點墨微陣列晶片

A Novel High-Throughput Proteomics Platform: The Micro-Western Array

Micro-Western Array（MWA）是一種新開發的高通量蛋白質體學系統，系統由奈升蛋白質微陣列點片機、水平乾燥式電泳和近紅外線微米尺度掃描器所組成。MWA系統的作用原理和傳統西方點墨技術相同，最多一次可以偵測384種蛋白質在6種樣品中的表現量和磷酸化狀態的變化，而且沒有現行蛋白質晶片交叉反應性的問題。MWA系統適合用於訊號傳遞路徑、生物系統或藥物作用機轉、篩選生物標誌以及驗證新開發抗體的專一性。我們相信MWA是系統生物和生物醫學研究的利器。 Micro-Western Array(MWA) , a novel proteomics platform, is composed of GeSim Nanoplotter protein array,GE Multiphor, and Licor Odyssey infrared scanner. MWA is a high-throughput Western blotting assay which isable to detect the abundance and phosphorylated status of 384 proteins in 6 samples simultaneously. Includingelectrophoresis procedure allows MWA to avoid antibody cross-reactivity, which is a common problem in proteinarray. MWA is most useful for signaling transduction studying, molecular mechanism investigation, biomarkerscreening, and antibody validation. MWA will be a very useful tool for systems biology and biomedical research.