利用氣相及液相層析法分離鑑別Morphine, Heroin, Lodeine Phosphate, Pentazocine等成分

DETERMINATION OF MORPHINE, HEROIN, LODEINE PHOSPHATE, AND PENTAZOCINE BY GAS CHROMATOGRAPHY AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

目前分離鑑別煙毒藥品：Morphine、Heroin、Codeine phosphate及Pentazocine；大都使用薄層層析法，一般採用以下幾種展開溶媒：一、MeOH：EtOH＝1：1。二、95％EtOH。三、MeOH：HOAC= 100：0.5，四、Benzene：Dioxane：EtOH：NH3＝50：40：5：5。五、Hexane：Ethylacetate：EtOH：NH3＝45：50：5：2。再以碘薰或噴以Iodoplatinate、Dragendorff's reagent呈色檢定，其結果雖準確但費時。 For now, TLC is the generally used method for the separation and identification of opium drugs. In this report, we used both GC and HPLC to separate and identify morphine HCl, Heroin, Codeine phosphate and pentazocine. The performance is good and efficient. 3% SE-30 and ov-17, 180 cm × 2 mm ID glass tubing, FID and 230°C were applied for the GC experiment while the experimental conditions for HPLC were (1). column: u-Bondapak C18; (2). Mobile phase: 5.8 g sodium Dioctyl-Sulfosuccinate in Methanol: Tetrahydrofuran: water= 650ml:20ml:330ml; (3). UV detector at 280nm; (4). flow rate: 1.5ml/min.