牛膝改善大鼠學習記憶障礙之研究

The Study of Achyranthes Bidentata on Learning and Memory Impairment in Rats

背景：阿茲海默症主要臨床症狀為記憶能力喪失，其病理特徵為老年斑，而老年斑主因類澱粉樣蛋白（amyloid β-peptide）沉積所致，基於此理論本實驗設計應用amyloid β peptide （Aβ1-40）來誘發阿茲海默症學習障礙之動物模型，並以水迷宮來評估中藥牛膝改善學習記憶障礙的作用。材料＆方法：本計畫以80%乙醇及水進行藥材萃取及分離，分別提取純化而製得懷牛膝皂苷提取物（ABS）及懷牛膝多醣體提取物（ABP）；後進行對Aβ1-40誘導大鼠記憶障礙改善作用之評估。結果：Aβ1-40可造成大鼠於水迷宮之操作障礙暨短期記憶與參考記憶之障礙。ABS於50 mg／kg可改善Aβ1-40側腦室輸注所導致水迷宮空間操作暨參考記憶能力之障礙；而ABP於100 mg／kg也能改善Aβ1-40側腦室輸注所導致水迷宮空間操作暨參考記憶能力之障礙。結論：懷牛膝之成分ABS及ABP能改善Aβ1-40誘導大鼠水迷宮學習記憶障礙，可供中醫藥臨床基礎研究參考。 Purpose: Alzheimer’s disease (AD) is characterized by neuropathological hallmarks such as senileplaque and neurofibrillary tangles caused by the amyloid β peptide (Aβ1-40). For this reason, we demonstratedthe role of the improve effect of Achyranthesbidentata against continuous intracisternal injection of Aβ1-40 in rats Materials & Methods: This present result obtained total saponins and total polysaccharides extracted from Actyranthes bidentate (ABS and ABP) by 80% ethanol and distilled water. We further investigated the effects of ABS and ABP on the memory function caused by Aβ1-40 in rats. Intracisternal injection of Aβ1-40 caused the dysfunction of memory function on water maze Results: Pretreatment with ABS at a dosage of 50 mg/kg or ABP at 100 mg/ kg reversed the dysfunction of memory function on water maze caused by intracisternal injection of Aβ1-40. Conclusions: ABS and ABP can improve the learning and memory impairment of rat water maze induced by Aβ1-40, which can be used as a reference for clinical basic research of traditional Chinese medicine.

Purpose: Alzheimer’s disease (AD) is characterized by neuropathological hallmarks such as senileplaque and neurofibrillary tangles caused by the amyloid β peptide (Aβ1-40). For this reason, we demonstratedthe role of the improve effect of Achyranthesbidentata against continuous intracisternal injection of Aβ1-40 in rats Materials & Methods: This present result obtained total saponins and total polysaccharides extracted from Actyranthes bidentate (ABS and ABP) by 80% ethanol and distilled water. We further investigated the effects of ABS and ABP on the memory function caused by Aβ1-40 in rats. Intracisternal injection of Aβ1-40 caused the dysfunction of memory function on water maze Results: Pretreatment with ABS at a dosage of 50 mg/kg or ABP at 100 mg/ kg reversed the dysfunction of memory function on water maze caused by intracisternal injection of Aβ1-40. Conclusions: ABS and ABP can improve the learning and memory impairment of rat water maze induced by Aβ1-40, which can be used as a reference for clinical basic research of traditional Chinese medicine.