結核分枝桿菌陽性病人治療前後之輔助T細胞細胞激素比較分析

The Comparison Study of Cytokine Expression by T Helper Cell between Pre- and Post-treatment MTB-positive Patients

結核病是致命的傳染病，故治療監控需快速檢驗協助。實驗研究被感染個體受結核桿菌刺激，反應Th細胞激發不同細胞激素含量分析，以輔助臨床診斷。病人收集群包含確診TB及治療2及6個月，潛伏性TB陽性和健康對照，分類五群以螢光微球抗原技術分析細胞激素IL-1β, IL-6, IL-4, IL-12, IL-17A, IFN-γ, TNF-α, TGFβ-1分泌量關係。結果發現IL-17在確診TB或潛伏群比健康群皆有顯著差異，且IL-6上升為對照痰中有菌指標，故這2項測值上升可做為感染或是開放性TB病人指標，做為加強治療或是持續隔離之依據。 Tuberculosis is a fatal contagious disease, by providing Immediate and quick test result for TB patients to make appropriate treatment and monitoring is important. The aim of the study is to analyze the T helper cell-associated cytokine expressed by TB patients, to see whether it can be used to aid the diagnosis of TB. Samples collected from confirmed TB patients were grouped into five categories 1) TB confirmed cases 2) TB patients after 2 months of treatment 3) TB patients after 6 months of treatment 4) latent TB patients 5) healthy donors. Cytokines levels of IL-1β, IL-6, IL-4, IL-12, IL-17A, IFN-γ, TNF-α, TGFβ-1 were analyzed respectively. Compared to healthy donors, the level of IL-17 is higher in TB confirmed patients and latent TB patients; on the other hand, increasing of IL-6 level indicates MTB in the sputum. In conclusion, the increasing of both cytokine levels mentioned above maybe used as the indicators to aid the clinician determine whether patients are in the period of MTB infection, in contagious status, need to receive intensive treatment or need to be isolated continuously.