| 中文摘要 |
本研究主要探討台灣二葉松（Pinus taiwanensis Hayata）木材抽出物之抗氧化及抗真菌活性。試驗結果顯示，台灣二葉松木材甲醇粗萃物各可溶部之DPPH自由基清除能力，以乙酸乙酯可溶部與正丁醇可溶部具有最佳抑制效果，其半數抑制濃度（IC50）分別為24.3μg/mL與24.7μg/mL。而亞鐵離子螯合能力則以水可溶部具有最佳抑制效果，其IC50為482.0μg/mL。另外，乙酸乙酯可溶部具有最高總酚類含量，其值為141.9 mg GAE/g。而在抗真菌活性方面，乙酸乙酯可溶部具有最佳抗真菌效果，其對白腐菌Trametes versicolor、Lenzites betulina以及褐腐菌Gloeophyllum trabeum、 Latetiporus sulphureus之IC50依序為320、296、43及34μg/mL。進一步分析乙酸乙酯可溶部中各次分離部（EA 1-EA 10）發現，EA 4、EA 5及EA 6均具有優異之抗真菌活性，且EA 6亦具有良好之抗氧化活性；而EA 2及EA 3則係同時兼具抗真菌活性及金屬螯合能力。綜合上述試驗結果確知台灣二葉松木材具有良好的抗氧化及抗真菌能力，極具開發應用之潛力。
The objective of this study is to evaluate antioxidant and antifungal activities of soluble fractions derived from the methanolic extract of Pinus taiwanensis wood. Various in vitro assays including DPPH radical scavenging activity, ferrous ion chelating ability, total phenolic contents, and agar plate test were carried out in this study. Results revealed that, among all the tested soluble fractions, the EtOAc and BuOH fractions showed the strongest DPPH radical scavenging activity with IC50 values of 24.3 and 24.7μg/mL, respectively. Additionally, the EtOAc fraction exhibited the highest total phenolic content (141.9 mg GAE/g), while the water fraction exhibited the highest ferrous ion chelating ability (IC50 = 482.0μg/mL). On the other hand, the EtOAc fraction exhibited the strongest antifungal activity on hypha growth of white-rot fungi, Trametes versicolor and Lenzites betulina, and brown-rot fungi, Gloeophyllum trabeum and Latetiporus sulphureus, with IC50 values of 320, 296, 43 and 34μg/mL, respectively. Furthermore, among 10 of EtOAc subfractions, the EA 4, EA 5, and EA 6 exhibited the strongest antifungal activity, and the EA 6 also exhibited good antioxidant activity. However, the EA 2 and EA 3 exhibited both antifungal and metal chelating ability. These results demonstrated that the methanolic extract of P. taiwanensis wood has excellent antioxidant and antifungal activities, indicating its potential applications are worthy of further investigations. |
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