COI基因條碼在臺灣產蝮蛇科及蝙蝠蛇科蛇種辨識上的應用

Application of Species Identification via Mitochondrial COI DNA Barcoding for Viperidae and Elapidae Species in Taiwan

利用聚合連鎖反應技術，針對臺灣產之6種蝮蛇科及5種蝙蝠蛇科蛇類粒線體COI基因進行局部擴增，獲得一段658個鹼基對之基因序列片段。以Kimura-2-parameter模式計算COI基因序列在種內與種間的遺傳距離，種內遺傳距離介於0.04 ～2.40%，種間的遺傳距離介於1.45～ 25.48%。另以序列鄰接法及最大簡約法方式建構系統發育樹，兩者呈現的聚類結果並未存在衝突，同種個體皆聚集在同一分支，顯示COI序列可應用於臺灣產蝮蛇科及蝙蝠蛇科之種間鑑別。 The PCR technique was used to amplify the partial mtDNA COI gene of 11 species of Viperidae and Elapidae snakes collected from Taiwan. The PCR products were sequenced and 658 bp of COI gene were obtained. The average Kimura-2-parameter genetic distance intra-species was 0.04 ~ 2.40%, and the inter-species was 1.45 ~ 25.48%. The phylogenetic trees estimated by neighbor-joining method and maximum-parsimony method obtained similar results. All 11 snake species showed monophyletic and every species could be discriminated clearly. It is suggested that the COI barcoding can be used to identify Viperidae and Elapidae snakes species in Taiwan.

The PCR technique was used to amplify the partial mtDNA COI gene of 11 species of Viperidae and Elapidae snakes collected from Taiwan. The PCR products were sequenced and 658 bp of COI gene were obtained. The average Kimura-2-parameter genetic distance intra-species was 0.04 ~ 2.40%, and the inter-species was 1.45 ~ 25.48%. The phylogenetic trees estimated by neighbor-joining method and maximum-parsimony method obtained similar results. All 11 snake species showed monophyletic and every species could be discriminated clearly. It is suggested that the COI barcoding can be used to identify Viperidae and Elapidae snakes species in Taiwan.