The regulatory effects of fish oil and chitosan on hepatic lipogenic signals in high-fat diet-induced obese rats   全文下載

The regulatory effects of fish oil and chitosan on hepatic lipogenic signals in high-fat diet-induced obese rats

The present study investigated the regulatory effects of fish oil and chitosan on the signals of hepatic lipid metabolismand the postulated mechanismin high-fat diet-induced obese rats. Diet supplementation of chitosan and fish oil efficiently suppressed the increased weights in body and livers of high-fat diet-fed rats. Supplementation of chitosan and fish oil significantly decreased the activities of hepatic lipid biosynthesis-related enzymes and efficiently regulated plasma lipoprotein homeostasis. Both chitosan and fish oil significantly ameliorated the alterations in the protein expressions of hepatic lipogenic transcription factors (LXRa and PPARa), and could also significantly regulate the downstream hepatic lipogenic genes (FAS, HMGCR, CYP7A1, FATP, FABP, AOX, and ABCA) expressions in high-fat diet-fed rats. These results suggest that both fish oil and chitosan exerts downregulative effects on hepatic lipid metabolism in high-fat diet-induced obese rats via the LXRa inhibition and PPARa activation, which further affect the expressions of hepatic lipogenesis-associated genes.