分散式液液微萃取結合高效液相層析法檢測藥品及體液中 Gemfibozil 含量   全文下載

Determination of Gemfibrozil in Drug Matrix and Human Biological Fluid by Dispersive Liquid-Liquid Microextraction with High Performance Liquid Chromatography

本研究開發一簡單、快速的分散式液液微萃取技術，結合高效液相層析法（HPLC）萃取、分析藥品、血漿及尿液中 gemfibrozil 含量。本方法以微升量之四氯化碳和甲醇分別作為萃取和分散溶劑。萃取和分散溶劑的種類、劑量、pH 值、檢體製備、萃取時間等都經仔細評估。最佳條件下，含量增加因子（enrichment factor）介於 9.2 - 9.3，萃取回收率介於 91.2 - 93.2%。濃度範圍 0.1 - 100 mg/L 時檢量線呈線性，偵測極限為 12.3 μg/L。10 重複檢測 gemfibrozil 含量之相對標準偏差（RSD）為 1.3%。本方法並經其他水樣藥品中 gemfibrozil 含量分析測試。

A simple and rapid dispersive liquid-liquid microextraction technique coupled with high performance liquid chromatography (HPLC) was developed for the extraction and analysis of gemfibrozil in drug matrix and biological fluids like human plasma and urine samples. In this method, carbon tetrachloride at microliter volume level and methanol were used as extraction and disperser solvents, respectively. The effects of factors including the type and volume of disperser and extraction solvents, pH and extraction time on the performance of the sample preparation were carefully evaluated. Under optimum conditions, the obtained enrichment factors were between 9.2 - 9.3 and extraction recoveries were high and ranged between 91.2 - 93.2%. The calibration curve was linear in the range of 0.1 - 100 mg/L with a detection limit of 12.3 µg/L. The relative standard deviation (RSD) for ten replicate measurements of gemfibrozil was 1.3%. The performance of the method was checked by the analysis of the gemfibrozil in presence of other drugs in water samples.