HPLC-DAD-ESI-MS 法對肉蓯蓉進行指紋圖譜和定量分析   全文下載

Chemical Fingerprint and Quantitative Analysis of Cistanche Deserticola by HPLC-DAD-ESI-MS

採用 HPLC-DAD-ESI-MS 法建立肉蓯蓉色譜指紋圖譜。分析了 11 批樣品，建立了肉蓯蓉的標準指紋圖譜。將 11 批樣品的指紋圖譜中的 8 個峰標記為特徵峰，通過與標準品的保留時間和質譜信息對比，進一步鑒定其結構。測定了 11 批樣品的相似度，結果表明不同來源的樣品具有相似的指紋圖譜。另外，採用 HPLC-DAD 法對肉蓯蓉中這 8 個成分進行含量測定。 8 個成分在線性範圍內呈現良好的線性關係（R2 > 0.9998），加樣回收率為93.65 - 109.79%。結果顯示本文建立的指紋圖譜結合定量分析的方法可以用來有效的控制肉蓯蓉的質量。

An HPLC-DAD-ESI-MS method was employed for the chromatographic fingerprint analysis of Cistanche deserticola (Roucongrong in Chinese). Eleven batches of samples were analyzed to establish the reference fingerprint of C. deserticola. Eight peaks in the fingerprint of all the 11 batches of samples were assigned as “characteristic peaks”, and identified by comparing their retention time and mass spectra with those of the reference substances. The similarity of the 11 batches of samples was evaluated by a simulative mean chromatogram. The results indicated that the samples from different origins shared similar HPLC fingerprints. In addition, an HPLC-DAD method was developed for simultaneous determination of the contents of the eight compounds in C. deserticola. All eight compounds showed good linear regression (R2 > 0.9998) within test ranges and the recovery of the method was in the range of 93.65 - 109.79%, indicating that the developed method combining chromatographic fingerprint with quantification analysis could readily be applied for the quality control of C. deserticola.