膜處理改變小柴胡湯水萃物的物化性、澄清度與抗氧化活性   全文下載

Physiochemical, Clarification, and Antioxidant Properties of Aqueous Extract of Xiao-Chai-Hu-Tang as Affected by Membrane Process

小柴胡湯為具抗氧化與保肝機能性的中國傳統草藥之一。但小柴胡湯不討喜之暗棕色與貯藏時有沉澱等問題，本研究評估膜處理對小柴胡湯萃取液澄清度與抗氧化能力之影響。以微過濾膜（0.45 μm）及四種超過濾膜（UK 200, UK 50, UP 20 and UK 10）處理。結果顯示因較少的總固形物可通過膜，因此透流液具較高的澄清度，而透流液含較多的活形成分如總酚、saikosaponins、glycyrrhizin 與 baicalin。透流液的抗氧化力包括清除自由基、還原力與總抗氧化力均高於原液，尤其是以 UK 10 膜處理者。整體而言，膜處理後之透流液不但有較高澄清度並含較高的活性成分之濃度，具開發為商業健康飲品之可行性。"

Xiao-chai-hu-tang (XCHT) is a traditional Chinese herbal medicine which has been demonstrated to exhibit antioxidant and hepato-protective functions. To reduce the unpleasant dark brown color and precipitates during storage, the performances of membrane process on the clarification and antioxidant capability of XCHT was evaluated. A membrane system equipped with either a microfiltration membrane (0.45 μm) or one of four ultrafiltration membranes (UK 200, UK 50, UP 20 and UK 10) was used. Our results indicated that less total solid was filtered through the membranes and thus a higher degree of clarification degree was achieved in permeates. Higher concentra-tions of active compounds, such as total phenolic compounds, saikosaponins, glycyrrhizin and baicalin, were detected in permeate using membranes of smaller pore-size of membranes. Antioxidant capabilities, including scavenging of free radicals, reducing power and total antioxidant activity of permeates, increased as compared to those of feed, particularly of permeates obtained with a UK 10 ultrafiltration membrane. Taken together, membrane process clarified aqueous herbal extract and selectively partitioned bioactive components in perme-ates, thus enhancing the feasibility of XCHT to be a commercial health drink product.