利用赤蘚紅結合光動力殺菌並使用綠色 LED 燈源對抗細菌及真菌   全文下載

Erythrosine-Mediated Photodynamic Inactivation of Bacteria and Yeast Using Green Light-Emitting Diode Light

赤蘚紅又稱為食用紅色色素3號，是一種的合成染料，已被廣泛使用在食品添加及牙菌斑顯示劑上。本研究的目的在評估赤蘚紅結合光動力殺菌於細菌及真菌上的可行性。細胞分別進行赤蘚紅處理或是有無醋酸的處理，實驗結果發現赤蘚紅不進行照光下不會對微生物生長造成影響。而進行光動力殺菌實驗中，赤蘚紅能有效的對抗革蘭氏陽性菌和酵母菌，而對革蘭氏陰性菌則效力較低。因此，為增加赤蘚紅於光動力殺菌之效益，於實驗中添加醋酸，發現可顯著降低革蘭氏陰性菌的生存。由此實驗可以證實，光動力殺菌與醋酸結合可增加赤蘚紅之效益。

Erythrosine, also known as FD&C Red No. 3, is a synthetic dye that has been used for food coloring and dental plaque detection. The objective of this study was to evaluate erythrosine as a viable photosensitizer in photodynamic inactivation (PDI) of bacteria and yeast. Cells were treated with erythrosine with or without acetic acid in the presence and absence of light. Erythrosine in the absence of light did not show any activity against the microorganisms. When treated with light, erythrosine was efficient in eradicating non-adherent (plank-tonic) Gram (+) bacteria and yeast. However, Gram (-) bacteria were less responsive to PDI. Addition of acetic acid was found to enhance the activity of erythrosine and significantly lower the survival of Gram (-) bacteria. This study demonstrated the potential applications of erythrosine in PDI and the potentiating effects of acetic acid.