HPLC 法同時測定光葉苦蕒菜中 6 種 Sesquiterpene Lactones 含量   全文下載

Simultaneous Determination of Sesquiterpene Lactones in Ixeris chinensis by HPLC

本研究開發逆相高效液相層析法同時檢測光葉苦?菜(Ixerischinensis)中6種SesquiterpeneLactones：3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-8β-hydroxy-4(15),10(14)11(13)-guaiatrien-12,6-olide(AR1);3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-4(15),10(14),11(13)-guaiatrien-12,6-olide(AR2);3β-(β-D-glucopyranosyloxy)-8β-(4-hydroxyphenyl)acetoxy-4(15),10(14),11(13)-guaiatrien-12,6-olide(AR3)3β-(β-D-glucopyranosyloxy)-8β-(2-hydroxy-3-methylbutanoyloxy)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide(AR4);3β-(β-D-glucopyranosyloxy)-4(15),10(14)11(13)-guaiatrien-12,6-olide(AR5)及3β-hydroxy-4(15),10(14),11(13)-guaiatrien-12,6-olide(AR6)。本方法以乙?：水(v/v)為流動相梯度洗脫，層析管柱為HypersilODS-2(250mm×4.6mm,5μm)，柱溫為30°C，檢測波長為238nm。本方法之線性、精密性、準確性及特異性均經評估，回收率95.85-98.19%，6種成分均呈良好線性關係（r>0.9993），具高度特異性及再現性（RSD%均小於2%），成功地應用於10個檢體的6種SesquiterpeneLactones檢測，結果顯示，本方法快速、準確、可靠，且可應用於光葉苦?菜定量分析。"

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of six sesqui-terpene lactones: 3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-8β-hydroxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR1) 3β-[3-(4-hydroxyphenyl)acetyl-β-D-glucopyranosyloxy]-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR2), 3β-(β-D-glucopyranosyloxy)-8β-(4-hydroxyphenyl)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR3), 3β-(β-D-glucopyranosyloxy)-8β-(2-hydroxy-3-methylbutanoyloxy)acetoxy-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR4), 3β-(β-D-glucopyranosyloxy)-4(15),10(14)11(13)-guaiatrien-12,6-olide (AR5), and 3β-hydroxy-4(15),10(14)11(13)- guaiatrien-12,6-olide (AR6) in the whole plant of Ixeris chinensis. The separation by gradient elution was performed on Hypersil ODS-2 column (250 mm × 4.6 mm, 5 µm) at 30°C with acetonitrile and water as the mobile phase, and monitored by absorbance at 238 nm. The parameters of linearity, precision, accuracy, and specificity of the method were evaluated. The recovery of the method is 95.85-98.19%, and linearity (r > 0.9993) was obtained for all sesquiterpene lactones. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.0%) were also achieved. This assay was successfully applied to the determination of six sesquiterpene lactones in ten samples. The results indicated that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for I. chinensis.