市售冷凍蝦仁之物種鑑定   全文下載

Identification of Species in Commercial Frozen Shrimp Meat in Taiwan

本研究收集台灣市售冷凍蝦仁樣品鑑定其物種。首先，採集10種普遍市售蝦各約20個新鮮樣品，以形態學方法鑑定。採用引子對UCYTB151F/ 270R進行細胞色素 b 基因的聚合?鏈反應（PCR），增幅出蝦樣品中401至407 bp的片段。比較基因定序，和使用Alu I 、Ssp I和Hae III限制?作用所產生的PCR-限制性片段長度多型性（RFLP），可以成功鑑定10種蝦。應用此PCR-RFLP方法於10種市售冷凍蝦肉之種類鑑定，顯示具有實用性。

Attempts were made to identify species in commercial frozen shrimp meat samples collected in Taiwan. First, approximately 20 fresh specimens of each of the 10 common commercial shrimp species were identified by morphological methods. A 401 to 407-bp fragment of the cytochrome b gene was amplified from the shrimp meat by polymerase chain reaction (PCR) using the primer pair UCYTB151F/270R. By comparing the gene sequences and PCR-restriction fragment length polymorphism (RFLP) maps obtained using the endonucleases Alu I, Ssp I and Hae III, the 10 species of shrimp could be successfully differentiated. The PCR-RFLP method was proved applicable for the species identification of 10 marketed shrimp samples.