Captopril 對黑色素生成效   全文下載

Effects of Captopril on Melanin Formation in B16 cells

本研究探討captopril對黑色素生成之影響。於B16黑色素腫瘤細胞模式中，captopril抑制酪胺酸?活性與其減少黑色素生成具有相關性，而captopril抑制酪胺酸?活性部分原因與其螯合銅離子有關。此外，captopril具有還原力與顯著抑制活性氧。另者，以逆轉錄聚合?鏈反應試驗，結果顯示captopril具有抑制酪胺酸?mRNA表現。綜合上述， captopril有抑制黑色素生成的特性，使其具有黑色素抑制劑潛在性。

The effect of captopril on melanin formation in B16 cells was investigated. In B16 intracellular model system, the effect of captopril on antityrosinase activity was found dose-dependent and correlated to its ability to suppress melanin formation. The copper chelation by captopril may in part be responsible for the inhibition of tyrosinase activity. Captopril also displayed a remarkable reducing ability and significantly inhibited ROS generation. Interestingly, captopril also significantly suppressed tyrosinase mRNA expression, as determined by reverse transcription-polymerase chain reaction (RT-PCR). Overall, the results showed that the protective effect of captopril makes it a potent inhibitor of melanin formation.