以高效能液相層析儀檢測與診斷豬隻黃疸肝臟中膽紅素的含量   全文下載

Determination and Identification of Bilirubin in Jaundiced Pig Liver by High Performance Liquid Chromatography

本研究以高效能液相層析儀開發豬肝中膽紅素含量的檢測方法，肝臟樣品經dimethyl sulfoxide及乙?萃取後以高效能液相層析儀分析。分析方法的準確度由添加3種不同濃度（2.5、5及10 μg/mL）膽紅素樣品之回收率表示，膽紅素回收率範圍為75.7 - 84.9%，而相同的添加濃度在同日內與異日間測試的精密分別為1.6 - 4.1%及2.4 - 3.9%。膽紅素的最低偵測極限值為0.3 μg/mL，分析一個樣品的時間為9分鐘。在此研究中統計了正常肝臟中膽紅素的濃度平均值與黃疸肝臟的區別，提供了科學的數據診斷黃疸症狀之膽紅素含量。此分析方法具有快速、靈敏、準確、良好的重複性與再現性，可成功的應用在豬肝中膽紅素的檢測。

A high performance liquid chromatography (HPLC) method was developed for the determination of bilirubin concentration in pig liver. The sample was extracted by sequential extraction with dimethyl sulfoxide and acetonitrile, followed by HPLC analysis. The accuracy of the analytical method was estimated by spiking pig liver samples with three different concentrations of bilirubin (2.5, 5 and 10 μg/mL) and a recovery of 75.7 - 84.9% was obtained, with corresponding intra-day and inter-day precisions of 1.6 - 4.1% and 2.4 - 3.9%, respectively. The limit of detection of bilirubin was 0.3 μg/mL and the analytical time for one sample was 9 min. In this study, the bilirubin concentration of normal and jaundiced pig livers was determined. It was concluded that the procedure could provide scientific data for detecting increased bilirubin concentration to identify jaundiced livers. The analytical method was found to be rapid, sensitive, accurate, repeatable and reproducible, and could be applied for the measurement of bilirubin concentration in pig liver.