以 Diethylcarbamazine（DEC）對蛋白質營養不良 C57BL/6J 小鼠肝細胞的作用效應   全文下載

Effects of Diethylcarbamazine (DEC) on Hepatocytes of c57bl/6j Mice Submitted to Protein Malnutrition

Diethylcarbamazine (DEC)雖是廣泛使用在治療絲蟲，其藥理潛力少被探索，在許多開發中國家和未開發國家常見蛋白質營養不良現象，其中有些是因淋巴絲蟲病影響導致，本研究目的是分析DEC在營養不良小鼠肝細胞上的作用。以48隻雄性C57BL/6小鼠分成兩組：對照組（C, D25和D50）及營養不良組（M,MD25和MD50），在誘發營養不良後，以濃度25 mg/kg和50 mg/kg的DEC溶液口服餵食治療12天，進行生化分析及光學顯微鏡、電子顯微鏡觀察肝細胞變化。就酵素劑量結果顯示，M組（2.52 ± 0.42 g/dL）比對照組（C組）（3.27 ± 0.43 g/dL）血清白蛋白濃度顯著減少， M組的組織學分析顯示其肝細胞明顯損害，然而， MD25和MD50這二組回復到基礎水平。M組，MD25和MD50和對照組（C組）比較，其鹼性磷酸鹽顯著增加。M組的組織學分析顯示有明顯的肝細胞損傷出現脂肪肝現象，MD25和MD50這二組脂肪減少，在MD25和MD50這二組的肝細胞超結構分析證實脂肪滴減少，有可能是DEC的影響，導致減少因營養不良造成的肝變化。"

Even though diethylcarbamazine (DEC) is widely used in the treatment for filaricide, its pharmacological potential is little explored. Protein malnutrition is highly incident in many populations in developing and under-developed countries and some of these populations are affected by lymphatic filariasis. The objective of the present study was to analyze the DEC effect in the hepatocytes of malnourished mice. Forty-eight male C57BL/6 mice were separated into groups: a control group (C, D25 and D50) and a malnourished group (M, MD25 and MD50). After being induced to malnutrition, the mice were submitted to 12 days of treatment with DEC solutions in concen-trations of 25 and 50 mg/kg which were orally administered. Biochemical analyses were performed and liver fragments were processed for light microscopy and transmission electron microscopy. For the enzymatic dosages, it was possible to observe a significant reduction in serum albumin in group M (2.52 ± 0.415 g/dL) when compared with the control group (C) (3.27 ± 0.427 g/dL) which received no treat-ment. The histological analysis of the M group showed evident hepatocellular damage. However, groups MD25 and MD50 returned to basal levels. Groups M, MD25 and MD50 presented a significant increase in alkaline phosphate when compared with the control group (C). Histological analysis of group M showed evident hepatic steatosis, which characterizes hepatocellular damage. Groups MD25 and MD50 showed a decrease in steatosis. An ultrastructural analysis of the hepatocytes in groups MD25 and MD50 confirmed a reduction of lipid droplets. It is possible that the DEC effects have contributed to the reduction of hepatic changes caused by malnutrition.