使用正交陣列獲致從梔子果實中萃取去羥梔子苷與梔子平的高效率與高重複性實驗條件   全文下載

Using Orthogonal Arrays to Obtain Efficient and Reproducible Extraction Conditions of Geniposide and Genipin in Gardenia Fruit with Liquid Chromatography-Mass Spectrometry Determinations

使用田口實驗設計法改進萃取梔子果實中成分的回收率，可獲致對去羥梔子?（geniposide）與梔子平（genipin）具有高效率與高重複性的萃取實驗條件。去羥梔子?是梔子中主要的環烯醚?類葡萄糖?，具有利膽效應，在動物體內會轉換成梔子平。本論文中，首先藉由實驗設計法準則，對主要的去羥梔子?與梔子平萃取條件，例如溶劑種類，溶劑酸鹼性，與煮沸時間等，經系統性探討後得到最高含量的萃取液，並使用液相層析串聯質譜術予以定量。由梔子Gardenia jasminoides Ellis 與Gardenia jasminoides Ellis var. grandiflora Nakai兩種果實磨成的粉末，皆被使用來研究。在本篇論文中，使用田口直交陣列可成功獲取最佳的操作條件，來由上述果實中萃取去羥梔子?與梔子平。這些設計方法僅需要經過九次的試誤實驗，就可以得到溶劑種類、煮沸時間、與溶劑酸度等三項探討因子的最佳條件。結果顯示，僅溶劑酸度會影響上述果實成分的萃取量，而溶劑種類與煮沸時間則對萃取量僅有些許影響。我們獲致的最高萃取量也與先前的文獻值相當。此外使用田口直交陣列，經過四次實驗試誤過程也可確認，使用精細的果實粉末可得到比使用粗糙的粉末獲致更有再現性的萃取結果。

The conditions to efficiently and reproducibly extract geniposide and genipin in Gardenia fruit powders were established using Taguchi experimental design approaches to improve extraction recovery. Geniposide, the major iridoid glycoside component of Gardenia fruits, which has been recognized to have choleretic effects, is transformed to Genipin in animals. In this study, the extraction conditions of geniposide and genipin such as solvent types, solvent acidities and boiling times were investigated under the guidance of experimental designs to obtain the highest content in extracts determined with liquid chromatography-mass spectrometry (LC-MS) methods. Herbal powders ground from Gardenia fruits, Gardenia jasminoides Ellis and Gardenia jasminoides Ellis var. grandiflora Nakai, were used. This paper has successfully demonstrated the use of Taguchi orthogonal arrays to obtain proper extraction conditions of geniposide and genipin in the above fruits. These design methods are capable of obtaining the optimum extraction conditions in only nine trials in which three factors, namely solvent type, boiling time and solvent acidity are investigated. The results show that solvent acidity is the influencing factor in achieving adequate contents and the other factors only have minor influences. The highest content obtained is similar to the reported data. In addition, using Taguchi orthogonal arrays within four experimental trials has identified that the extraction of ground fine powders results in more reproducible recovery than that of coarse ones.