金奈米粒子為基礎的共振光散射法檢測 Phenothiazine 類藥品   全文下載

Gold Nanoparticles-Based Resonance Light Scattering Method for the Determination of Phenothiazine Drugs

本研究開發一種新的金奈米粒子為基礎的共振光散射法（resonance light scattering, RLS）用於檢測2種Phenothiazine類藥品，thioridazine hydro-chloride和chlorpromazine hydrochloride。將該類藥品加入金奈米粒子溶液中，酸性介質會導致奈米粒子聚集，在波長400 nm時，依序引起顯著增加之共振光散射強度，利用紫外光／可見光光譜儀及穿透式電子顯微鏡將這些聚合物分類。實驗之重要因子如金奈米粒子之pH值、濃度及共振光散射訊號培養時間等均經研究並優化。最優化條件下，thioridazine hydrochloride及chlorpromazine hydrochloride分別於濃度範圍3.6 - 36 and 3.5 - 30 ng/mL時呈線性，偵測極限分別為1.1和1.0 ng/mL。實驗結果顯示，金奈米粒子為基礎的共振光散射法應用於檢測製劑及人血漿中phenothiazine類藥品，既靈敏又簡單。"

In this work, a new method was developed for the determination of two phenothiazine drugs, namely thioridazine hydrochloride and chlorpromazine hydrochloride using gold nanoparticles-based resonance light scattering (RLS) technique. The addition of these drugs to gold nanoparticles solution in acidic medium leads to the aggregation of nanoparticles, which, in turn, causes a marked enhancement in RLS intensity at 400 nm. The aggregates were characterized by UV-Vis spectroscopy and transmission electron microscopy. The effects of important experimental factors such as pH, concentration of gold nanoparticles and incubation time on RLS signal were inves-tigated and optimized. Under the optimum conditions, the calibration graphs were linear in the range of 3.6 - 36 and 3.5 - 30 ng/mL, for thioridazine hydrochloride and chlorpromazine hydrochloride, respectively with corresponding limits of detection of 1.1 and 1.0 ng/mL. The results indicate that the nanogold-based RLS is a very sensitive and simple method for the determination of phenothiazine drugs in pharmaceutical samples and human plasma.