台灣產梅子產品抗過敏活性及定性定量分析   全文下載

Qualitative and Quantitative Analyses of the Anti-Allergic Constituent of Commercial Prunus mume Products in Taiwan

梅精為梅子經壓榨萃取後之高濃度黑色糖漿產物。在亞洲，梅精為普遍的健康補充品之一。其活性成分5-hydroxymethylfurfural (HMF) (1)，在RBL-2H3細胞株中，可抑制β-hexosaminidase degranulation並具有濃度依存性。為了分析梅精中的HMF，本實驗利用HPLC針對23項市售梅精產品進行HMF的定性定量分析；包含10項台灣梅精產品、3項日本梅精產品、5項特殊製程梅精產品、2項市售梅子飲品、1項新鮮成熟梅子萃取物及兩個經加熱萃取之梅子萃取物。經高效能層析儀圖譜分析指出，這些市售產品HMF含量不一。其中，梅精水萃物有較好的HMF產率。因此，我們推測不同原料來源、萃取方法及製程會影響HMF之含量。

The juice extracted from Prunus mume (Mei-Gin) is in the form of a highly concentrated dark black syrup. It is a common health supplement in Asia. The active component, 5-hydroxymethylfurfural (HMF), manifested a concentration-dependent effect on the inhibition of β-hexosaminidase degranulation upon antigen-sensitized RBL-2H3 cells. In order to investigate HMF (1) of P. mume extract, HMF was isolated and the HPLC profiles of 23 commercial Mei-Gin products (6 types) were qualitatively and quantitatively analyzed for HMF content. The samples included 10 Taiwanese Mei-Gin products, 3 Japanese Mei-Gin products, 5 specific-processing Mei-Gin products, 2 commercial plum drinks, 1 fresh mature plum extract, and 2 heat-processed samples. The HPLC profiles showed that the commercial products contained various amounts of HMF. Among them, the water extract of P. mume showed better yield of HMF. The yields from such products may differ due to different Mei-Gin original materials and different extraction and manufacturing procedures.